ABSTRACT
OBJECTIVES@#To estimate lung volume in normal population by a combination of multi-slice spiral CT and Pulmo software.@*METHODS@#Scans of the lung were performed on 45 normal individuals using Emotion 16-slice spiral CT and Pulmo software. A measurement instrument of work station was used to measure the vertical diameter of left and right lungs, the maximum anteroposterior and transverse diameters at the layers of apex pulmonis 1 cm below, arcus aortae, subcarinal and diaphragmatic dome, and the lung volume. Regression analysis was performed on normal lung volume and each measured parameter by SPSS 20.0 and an optimal model was selected.@*RESULTS@#The goodness of fit between the best curvilinear equations of the normal bilateral lung volumes was 0.981. The goodness of fit between the curvilinear equations of the normal bilateral lung volume and the product of the vertical diameter and the maximum transverse diameter of diaphragmatic dome were 0.977 and 0.972, respectively. Fifteen cases were selected to make a retrospective test on the 3 models. No significant difference was found between the estimated and measured with Pulmo software lung volumes.@*CONCLUSIONS@#The normal lung volume before injury can be estimated by the curvilinear equations established in this study. The percentage of lung compression can be measured based on the volume of compressed lung, which can provide a reference for the identification of lung compression degree.
Subject(s)
Humans , Lung/diagnostic imaging , Retrospective Studies , Software , Tomography, Spiral Computed , Tomography, X-Ray ComputedABSTRACT
OBJECTIVES@#To observe the changes of expression of α-synuclein （α-syn） and neuronal apoptosis in brain cortex of acute alcoholism rats and to explore the mechanism of the damage caused by ethanol to the neurons.@*METHODS@#The model of acute alcoholism rat was established by 50% alcohol gavage. The α-syn and caspase-3 were detected by immunohistochemical staining and imaging analysis at 1 h, 3 h, 6 h and 12 h after acute alcoholism. The number of positive cell and mean of optical density were detected and the trend change was analyzed. The variance analysis and t-test were also performed.@*RESULTS@#The number of α-syn positive cell and average optical density in brain cortex of acute alcoholism rat increased significantly and peaked at 6 hour with a following slight decrease at 12 h, but still higher than the groups at 1 h and 3 h. Within 12 hours after poisoning, the number of caspase-3 positive cell and average optical density in brain cortex of rats gradually increased.@*CONCLUSIONS@#The abnormal aggregation of α-syn caused by brain edema and hypoxia may participate the early stage of neuronal apoptosis in brain cortex after acute alcoholism.
Subject(s)
Animals , Rats , Alcoholism/pathology , Apoptosis , Brain Edema/pathology , Caspase 3/metabolism , Cerebral Cortex/pathology , Ethanol , Hypoxia/pathology , Neurons/pathology , alpha-Synuclein/metabolismABSTRACT
Medical tangles caused by the death of women and infants in perinatal period are very normal in the forensic appraisal. The author collected and analyzed 49 cases of these tangles from many aspects, such as sex and age of the dead, hospital,information of autopsy, fault of medical action and so on,and discovered the normal causes of death, medical action's effects and the causes of tangle. It would be useful to the forensic appraisal, settlement and prevention of these medical tangles.
Subject(s)
Adult , Female , Humans , Infant, Newborn , Male , Pregnancy , Young Adult , Asphyxia Neonatorum/mortality , Autopsy , Cause of Death , Forensic Pathology , Malpractice/legislation & jurisprudence , Maternal Mortality , Postpartum Hemorrhage/mortality , Respiratory Tract Diseases/mortality , Retrospective StudiesABSTRACT
OBJECTIVE@#To observe the changes of DNA fragmentation and its quantity along with the time of injury in nerve cells after brain contusion.@*METHODS@#The model of brain contusion caused by free drop hammer was established. TUNEL and Feulgen's DNA staining conjoined with image analysis technique were used for exploration.@*RESULTS@#With the gradually rising of DNA fragmentation, DNA quantity was declining in the brain tissue after contusion.@*CONCLUSION@#TUNEL and Feulgen's DNA staining conjoined with image analysis technique could be utilized in the timing of brain injury and provide a new approach for this issue.